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human fetal lung stromal lines wi38  (ATCC)


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    ATCC human fetal lung stromal lines wi38
    In vitro screening of a NMA library for potential senotherapeutics. A) A schematic representation of the procedure for human cell‐based screening of a NMA library composed of 66 naturally‐derived agents. Screening was first performed for all natural compounds for senomorphics, with the efficacy of potential candidates further validated in multiple human stromal cell lines including PSC27, <t>WI38</t> and IMR90. [ <xref ref-type= 85 ] B) Experimental outputs of senolytics screening after pharmacological treatments in vitro. Natural compounds were assayed by incubation for 3 days at a concentration of 3 µg/mL/agent (ABT‐263 at 1.25 µM) with 5.0x10 3 cells. Each hit denoted by a blue dot is shown based on the effect to selectively kill SEN but not CTRL cells, and represents the mean of 3 biological replicates. The red‐edged rectangular region shows candidates of senolytics. Note, only ABT‐263 and procyanidin C1 (PCC1), the positive controls, fell in this specific region. C) Evaluation of the efficacy of natural compounds as senolytics in SEN and CTRL counterpart cells. (D‐E) Assessment of the efficacy of natural compounds (D, group A, E, group B) by analyzing the expression of a canonical SASP soluble factor CXCL8. For all datasets, samples were examined after treatment with individual agents in culture for 3 days. NMA, natural medicinal agent. CTRL, control. SEN, senescent. TIS, therapy‐induced senescence. Data in C, D and E are shown as mean ± SD and representative of 3 independent biological replicates. P values were calculated by Student's t‐ tests. P > 0.05, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. " width="250" height="auto" />
    Human Fetal Lung Stromal Lines Wi38, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 3132 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+fetal+lung+stromal+lines+wi38/WI-38/pmc12120719-270-2-12
    Average 99 stars, based on 3132 article reviews
    human fetal lung stromal lines wi38 - by Bioz Stars, 2026-10
    99/100 stars

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    1) Product Images from "Targeting Senescence with Apigenin Improves Chemotherapeutic Efficacy and Ameliorates Age‐Related Conditions in Mice"

    Article Title: Targeting Senescence with Apigenin Improves Chemotherapeutic Efficacy and Ameliorates Age‐Related Conditions in Mice

    Journal: Advanced Science

    doi: 10.1002/advs.202412950

    In vitro screening of a NMA library for potential senotherapeutics. A) A schematic representation of the procedure for human cell‐based screening of a NMA library composed of 66 naturally‐derived agents. Screening was first performed for all natural compounds for senomorphics, with the efficacy of potential candidates further validated in multiple human stromal cell lines including PSC27, WI38 and IMR90. [ <xref ref-type= 85 ] B) Experimental outputs of senolytics screening after pharmacological treatments in vitro. Natural compounds were assayed by incubation for 3 days at a concentration of 3 µg/mL/agent (ABT‐263 at 1.25 µM) with 5.0x10 3 cells. Each hit denoted by a blue dot is shown based on the effect to selectively kill SEN but not CTRL cells, and represents the mean of 3 biological replicates. The red‐edged rectangular region shows candidates of senolytics. Note, only ABT‐263 and procyanidin C1 (PCC1), the positive controls, fell in this specific region. C) Evaluation of the efficacy of natural compounds as senolytics in SEN and CTRL counterpart cells. (D‐E) Assessment of the efficacy of natural compounds (D, group A, E, group B) by analyzing the expression of a canonical SASP soluble factor CXCL8. For all datasets, samples were examined after treatment with individual agents in culture for 3 days. NMA, natural medicinal agent. CTRL, control. SEN, senescent. TIS, therapy‐induced senescence. Data in C, D and E are shown as mean ± SD and representative of 3 independent biological replicates. P values were calculated by Student's t‐ tests. P > 0.05, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. " title="... in multiple human stromal cell lines including PSC27, WI38 and IMR90. [ 85 ..." property="contentUrl" width="100%" height="100%"/>
    Figure Legend Snippet: In vitro screening of a NMA library for potential senotherapeutics. A) A schematic representation of the procedure for human cell‐based screening of a NMA library composed of 66 naturally‐derived agents. Screening was first performed for all natural compounds for senomorphics, with the efficacy of potential candidates further validated in multiple human stromal cell lines including PSC27, WI38 and IMR90. [ 85 ] B) Experimental outputs of senolytics screening after pharmacological treatments in vitro. Natural compounds were assayed by incubation for 3 days at a concentration of 3 µg/mL/agent (ABT‐263 at 1.25 µM) with 5.0x10 3 cells. Each hit denoted by a blue dot is shown based on the effect to selectively kill SEN but not CTRL cells, and represents the mean of 3 biological replicates. The red‐edged rectangular region shows candidates of senolytics. Note, only ABT‐263 and procyanidin C1 (PCC1), the positive controls, fell in this specific region. C) Evaluation of the efficacy of natural compounds as senolytics in SEN and CTRL counterpart cells. (D‐E) Assessment of the efficacy of natural compounds (D, group A, E, group B) by analyzing the expression of a canonical SASP soluble factor CXCL8. For all datasets, samples were examined after treatment with individual agents in culture for 3 days. NMA, natural medicinal agent. CTRL, control. SEN, senescent. TIS, therapy‐induced senescence. Data in C, D and E are shown as mean ± SD and representative of 3 independent biological replicates. P values were calculated by Student's t‐ tests. P > 0.05, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Techniques Used: In Vitro, Derivative Assay, Incubation, Concentration Assay, Expressing, Control

    Related Articles

    Cell Culture:

    Article Title: Senescent cells develop PDK4-dependent hypercatabolism and form an acidic microenvironment to drive cancer resistance
    Article Snippet: .. Human fetal lung stromal lines WI38 and HFL1, and foreskin stromal line BJ were from ATCC and cultured with F-12K medium supplemented with 10% FBS. .. Prostate cancer epithelial cell lines PC3, DU145, LNCaP and VCaP (ATCC) were routinely cultured with RPMI 1640 (10% FBS).

    Article Title: Rutin is a potent senomorphic agent to target senescent cells and can improve chemotherapeutic efficacy.
    Article Snippet: .. Human fetal lung stromal lines WI38 and HFL1, and foreskin stromal line BJ were from ATCC and cultured with F- 12 K medium supplemented with 10% FBS. .. Prostate cancer epithelial cell lines PC3, DU145, and LNCaP, as well as breast cancer epithelial cell lines MDA- MB- 231, SUM159, T47D, and MCF- 7 (ATCC) were routinely cultured with RPMI 1640 (10% FBS).

    Article Title: Repurposing the plant-derived compound apigenin for senomorphic effect in antiaging pipelines
    Article Snippet: .. Human fetal lung stromal lines WI38 and IMR90 were from ATCC and cultured with F-12K medium supplemented with 10% FBS. .. Prostate cancer epithelial cell lines PC3, DU145 and LNCaP, and human embryonic kidney line 293T were from ATCC and routinely cultured with RPMI 1640 (10% FBS).

    Article Title: Rutin is a potent senomorphic agent to target senescent cells and can improve chemotherapeutic efficacy
    Article Snippet: .. Human fetal lung stromal lines WI38 and HFL1, and foreskin stromal line BJ were from ATCC and cultured with F‐12 K medium supplemented with 10% FBS. .. Prostate cancer epithelial cell lines PC3, DU145, and LNCaP, as well as breast cancer epithelial cell lines MDA‐MB‐231, SUM159, T47D, and MCF‐7 (ATCC) were routinely cultured with RPMI 1640 (10% FBS).

    Article Title: Targeting Senescence with Apigenin Improves Chemotherapeutic Efficacy and Ameliorates Age‐Related Conditions in Mice
    Article Snippet: .. [ ] Human fetal lung stromal lines WI38 and IMR90 were from ATCC and cultured with F‐12K medium supplemented with 10% FBS. .. Prostate cancer epithelial cell lines PC3, DU145, and LNCaP and the human embryonic kidney line 293T were from ATCC and cultured with RPMI 1640 (10% FBS).

    Article Title: PDK4-dependent hypercatabolism and lactate production of senescent cells promotes cancer malignancy
    Article Snippet: .. Human fetal lung stromal lines WI38, IMR90 and HFL1, foreskin stromal line BJ and human embryonic kidney line 293T were from ATCC and cultured with F-12K medium supplemented with 10% FBS. .. Prostate cancer epithelial cell lines PC3, DU145, LNCaP and VCaP, as well as breast cancer cell line MDA-MB-231 (ATCC) were routinely cultured with RPMI 1640 (10% FBS).

    Article Title: Targeting Senescence with Apigenin Improves Chemotherapeutic Efficacy and Ameliorates Age-Related Conditions in Mice.
    Article Snippet: .. [26] Human fetal lung stromal lines WI38 and IMR90 were from ATCC and cultured with F-12K medium supplemented with 10% FBS. .. Prostate cancer epithelial cell lines PC3, DU145, and LNCaP and the human embryonic kidney line 293T were from ATCC and cultured with RPMI 1640 (10% FBS).

    Article Title: Senescent cells develop PDK4-dependent hypercatabolism and produce lactate to promote cancer resistance and aging
    Article Snippet: .. Human fetal lung stromal lines WI38 and HFL1, and foreskin stromal line BJ were from ATCC and cultured with F-12K medium supplemented with 10% FBS. .. Prostate cancer epithelial cell lines PC3, DU145, LNCaP and VCaP, as well as breast cancer cell line MDAMB-231 (ATCC) were routinely cultured with RPMI 1640 (10% FBS).



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    human fetal lung stromal lines wi38  (ATCC)
    99
    ATCC human fetal lung stromal lines wi38
    In vitro screening of a NMA library for potential senotherapeutics. A) A schematic representation of the procedure for human cell‐based screening of a NMA library composed of 66 naturally‐derived agents. Screening was first performed for all natural compounds for senomorphics, with the efficacy of potential candidates further validated in multiple human stromal cell lines including PSC27, <t>WI38</t> and IMR90. [ <xref ref-type= 85 ] B) Experimental outputs of senolytics screening after pharmacological treatments in vitro. Natural compounds were assayed by incubation for 3 days at a concentration of 3 µg/mL/agent (ABT‐263 at 1.25 µM) with 5.0x10 3 cells. Each hit denoted by a blue dot is shown based on the effect to selectively kill SEN but not CTRL cells, and represents the mean of 3 biological replicates. The red‐edged rectangular region shows candidates of senolytics. Note, only ABT‐263 and procyanidin C1 (PCC1), the positive controls, fell in this specific region. C) Evaluation of the efficacy of natural compounds as senolytics in SEN and CTRL counterpart cells. (D‐E) Assessment of the efficacy of natural compounds (D, group A, E, group B) by analyzing the expression of a canonical SASP soluble factor CXCL8. For all datasets, samples were examined after treatment with individual agents in culture for 3 days. NMA, natural medicinal agent. CTRL, control. SEN, senescent. TIS, therapy‐induced senescence. Data in C, D and E are shown as mean ± SD and representative of 3 independent biological replicates. P values were calculated by Student's t‐ tests. P > 0.05, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. " width="250" height="auto" />
    Human Fetal Lung Stromal Lines Wi38, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+fetal+lung+stromal+lines+wi38/WI-38/pmc12120719-270-2-12
    Average 99 stars, based on 1 article reviews
    human fetal lung stromal lines wi38 - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier

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    In vitro screening of a NMA library for potential senotherapeutics. A) A schematic representation of the procedure for human cell‐based screening of a NMA library composed of 66 naturally‐derived agents. Screening was first performed for all natural compounds for senomorphics, with the efficacy of potential candidates further validated in multiple human stromal cell lines including PSC27, WI38 and IMR90. [ <xref ref-type= 85 ] B) Experimental outputs of senolytics screening after pharmacological treatments in vitro. Natural compounds were assayed by incubation for 3 days at a concentration of 3 µg/mL/agent (ABT‐263 at 1.25 µM) with 5.0x10 3 cells. Each hit denoted by a blue dot is shown based on the effect to selectively kill SEN but not CTRL cells, and represents the mean of 3 biological replicates. The red‐edged rectangular region shows candidates of senolytics. Note, only ABT‐263 and procyanidin C1 (PCC1), the positive controls, fell in this specific region. C) Evaluation of the efficacy of natural compounds as senolytics in SEN and CTRL counterpart cells. (D‐E) Assessment of the efficacy of natural compounds (D, group A, E, group B) by analyzing the expression of a canonical SASP soluble factor CXCL8. For all datasets, samples were examined after treatment with individual agents in culture for 3 days. NMA, natural medicinal agent. CTRL, control. SEN, senescent. TIS, therapy‐induced senescence. Data in C, D and E are shown as mean ± SD and representative of 3 independent biological replicates. P values were calculated by Student's t‐ tests. P > 0.05, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. " width="100%" height="100%">

    Journal: Advanced Science

    Article Title: Targeting Senescence with Apigenin Improves Chemotherapeutic Efficacy and Ameliorates Age‐Related Conditions in Mice

    doi: 10.1002/advs.202412950

    Figure Lengend Snippet: In vitro screening of a NMA library for potential senotherapeutics. A) A schematic representation of the procedure for human cell‐based screening of a NMA library composed of 66 naturally‐derived agents. Screening was first performed for all natural compounds for senomorphics, with the efficacy of potential candidates further validated in multiple human stromal cell lines including PSC27, WI38 and IMR90. [ 85 ] B) Experimental outputs of senolytics screening after pharmacological treatments in vitro. Natural compounds were assayed by incubation for 3 days at a concentration of 3 µg/mL/agent (ABT‐263 at 1.25 µM) with 5.0x10 3 cells. Each hit denoted by a blue dot is shown based on the effect to selectively kill SEN but not CTRL cells, and represents the mean of 3 biological replicates. The red‐edged rectangular region shows candidates of senolytics. Note, only ABT‐263 and procyanidin C1 (PCC1), the positive controls, fell in this specific region. C) Evaluation of the efficacy of natural compounds as senolytics in SEN and CTRL counterpart cells. (D‐E) Assessment of the efficacy of natural compounds (D, group A, E, group B) by analyzing the expression of a canonical SASP soluble factor CXCL8. For all datasets, samples were examined after treatment with individual agents in culture for 3 days. NMA, natural medicinal agent. CTRL, control. SEN, senescent. TIS, therapy‐induced senescence. Data in C, D and E are shown as mean ± SD and representative of 3 independent biological replicates. P values were calculated by Student's t‐ tests. P > 0.05, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Article Snippet: [ ] Human fetal lung stromal lines WI38 and IMR90 were from ATCC and cultured with F‐12K medium supplemented with 10% FBS.

    Techniques: In Vitro, Derivative Assay, Incubation, Concentration Assay, Expressing, Control